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Call To Power 2 Cradle 3+ mod in progress: https://apolyton.net/forum/other-games/call-to-power-2/ctp2-creation/9437883-making-cradle-3-fully-compatible-with-the-apolyton-edition
No, I'm not saying you're unproductive. I was just curious. BTW even though privately funded research might be a bit better supervised, there's no guarantee that it's going to be more productive because of that. So you might as well have long coffee breaks
If they want to snort my primers, they can go explain where the £250 of grant money went. Synthesising oligonucleotides is silly expensive
My lab had to tell me why I can't use my GFP carrying adenovirus protocol to make myself glow in th dark.
Exult in your existence, because that very process has blundered unwittingly on its own negation. Only a small, local negation, to be sure: only one species, and only a minority of that species; but there lies hope. [...] Stand tall, Bipedal Ape. The shark may outswim you, the cheetah outrun you, the swift outfly you, the capuchin outclimb you, the elephant outpower you, the redwood outlast you. But you have the biggest gifts of all: the gift of understanding the ruthlessly cruel process that gave us all existence [and the] gift of revulsion against its implications.
-Richard Dawkins
Exult in your existence, because that very process has blundered unwittingly on its own negation. Only a small, local negation, to be sure: only one species, and only a minority of that species; but there lies hope. [...] Stand tall, Bipedal Ape. The shark may outswim you, the cheetah outrun you, the swift outfly you, the capuchin outclimb you, the elephant outpower you, the redwood outlast you. But you have the biggest gifts of all: the gift of understanding the ruthlessly cruel process that gave us all existence [and the] gift of revulsion against its implications.
-Richard Dawkins
Oooh, you doing a Ph.D.? Thought you were an undergrad. Where and in what?
Exult in your existence, because that very process has blundered unwittingly on its own negation. Only a small, local negation, to be sure: only one species, and only a minority of that species; but there lies hope. [...] Stand tall, Bipedal Ape. The shark may outswim you, the cheetah outrun you, the swift outfly you, the capuchin outclimb you, the elephant outpower you, the redwood outlast you. But you have the biggest gifts of all: the gift of understanding the ruthlessly cruel process that gave us all existence [and the] gift of revulsion against its implications.
-Richard Dawkins
No, 2nd year Chemist, at Durham. I'm on a masters as it is, but I'm probably going to change to a BS.c, as I want to do a PGCE. I hate working in labs. So you're safe from me stealing your research
You just wasted six ... no, seven ... seconds of your life reading this sentence.
And you prefer a room full of snot nosed kids to lab work? At least in the lab if the cells piss me off, I can kill them. Try doing that with kids.
Exult in your existence, because that very process has blundered unwittingly on its own negation. Only a small, local negation, to be sure: only one species, and only a minority of that species; but there lies hope. [...] Stand tall, Bipedal Ape. The shark may outswim you, the cheetah outrun you, the swift outfly you, the capuchin outclimb you, the elephant outpower you, the redwood outlast you. But you have the biggest gifts of all: the gift of understanding the ruthlessly cruel process that gave us all existence [and the] gift of revulsion against its implications.
-Richard Dawkins
SC it sounds like you're doing something similar to my GF ... she's an immunology Ph.D. student (also government funded, yay government money) and uses GFPs or something similar all the time She does mostly FACS stuff, which would probably be a bit more complex than GFP since you need multiple colors I think (and can normally be done ex vivo), but I know she's had to use GFP on occasion to follow her CD4 T cells around.
Do you have long nights up to 4am running tube after tube of crap all night long also
<Reverend> IRC is just multiplayer notepad.
I like your SNOOPY POSTER! - While you Wait quote.
Originally posted by snoopy369
SC it sounds like you're doing something similar to my GF ... she's an immunology Ph.D. student (also government funded, yay government money) and uses GFPs or something similar all the time She does mostly FACS stuff, which would probably be a bit more complex than GFP since you need multiple colors I think (and can normally be done ex vivo), but I know she's had to use GFP on occasion to follow her CD4 T cells around.
Do you have long nights up to 4am running tube after tube of crap all night long also
Well, I'm involved with the proteins that synthesis DNA in the presence of damage, specifically their regulation. It sorta crosses over into immunology since a few of these proteins are indicated to be involved in the generation of antibodies. GFP's become the universal method for tagging proteins in cells since its (relatively) easy, non-toxic and pretty.
I don't have the late nights yet but I've only just started. I'll have the weird hours soon enough.
Exult in your existence, because that very process has blundered unwittingly on its own negation. Only a small, local negation, to be sure: only one species, and only a minority of that species; but there lies hope. [...] Stand tall, Bipedal Ape. The shark may outswim you, the cheetah outrun you, the swift outfly you, the capuchin outclimb you, the elephant outpower you, the redwood outlast you. But you have the biggest gifts of all: the gift of understanding the ruthlessly cruel process that gave us all existence [and the] gift of revulsion against its implications.
-Richard Dawkins
Yeah, she tags them sometimes for that reason (I think it's one of the easiest ways to identify a cell that has the protein she's interested in).
Just be glad you're dealing with something in short time periods ... she works on memory cells largely, so her experiments all have 90 or 120 or even 180 day timeframes, and if that 30 day timepoint falls on Christmas, or on a day she is very very busy on already, she still has to do the 20-40 hour experiment... She is getting better at planning that stuff, but still...
Have you ever used MACS (Magnetic asssited cell sorting)? It's one of the funniest setups i've ever seen (and so much cheaper and easier than FACS), though how they change $150 or something for the metal stand I will never know ...
<Reverend> IRC is just multiplayer notepad.
I like your SNOOPY POSTER! - While you Wait quote.
She does mostly FACS stuff, which would probably be a bit more complex than GFP since you need multiple colors I think (and can normally be done ex vivo), but I know she's had to use GFP on occasion to follow her CD4 T cells around. 
She is getting better at planning that stuff, but still...
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