I've only had a few times where I got a really good read on somebody because of a physical reaction. It's really amazing when it happens.
One of the times it was on a raised pot in a NL tourney. Only me and one other guy in the pot. I'm holding A-2 (forgive me; there were only 3 people at the table at this point). Flop comes A-Q-7. I bet slightly more than the pot, he comes back over the top of me for all his chips (we were holding similar stacks). I looked at him as I said "I'm holding an Ace. What do you think about that". He said "I don't know", but his voice broke with tension as he said it. It was clear as day to me that I should call him at that point, and I was right. He was holding A-5. We were almost guaranteed to split the pot, and I thus had odds to call him. As it turns out, I spiked a 2 and took the whole thing, but the important part was that his weakness was as clear as day in his voice. I knew he either had a really weak ace or a queen. No way was he in strength at that point.
One of the times it was on a raised pot in a NL tourney. Only me and one other guy in the pot. I'm holding A-2 (forgive me; there were only 3 people at the table at this point). Flop comes A-Q-7. I bet slightly more than the pot, he comes back over the top of me for all his chips (we were holding similar stacks). I looked at him as I said "I'm holding an Ace. What do you think about that". He said "I don't know", but his voice broke with tension as he said it. It was clear as day to me that I should call him at that point, and I was right. He was holding A-5. We were almost guaranteed to split the pot, and I thus had odds to call him. As it turns out, I spiked a 2 and took the whole thing, but the important part was that his weakness was as clear as day in his voice. I knew he either had a really weak ace or a queen. No way was he in strength at that point.
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